A screening method for cell wall inhibitors using a D-cycloserine hypersensitive mutant.
نویسندگان
چکیده
Large numbers of antibiotics have been discovered in the past 40 years and manymethods have been employed for discovery of novel antibiotics. Oneof them is the use of mutants that are highly sensitive to antibiotics. Recently /2-lactam antibiotic-hypersensitive mutants of Escherichia colil) and Pseudomonas aemginosa2) and an aminoglycoside-hypersensitive mutant of Klebsiella pneumoniaez) have been used in screening for new antibiotics. D-Cycloserine (DCS) has a broad antibacterial spectrum0 and acts on alanine racemase (EC 5.1.1.1) and D-alanine: D-alanine ligase (EC 6.3.2.4), which are involved in synthesis of the
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عنوان ژورنال:
- The Journal of antibiotics
دوره 41 6 شماره
صفحات -
تاریخ انتشار 1988